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TitleCarbendazim dissipation in the biomixture of on-farm biopurification systems and its effect on microbial communities
Author(s)Tortella, G.
Mella-Herrera, R.
Sousa, D. Z.
Rubilar, Olga
Briceño, G.
Parra, L.
Diez, M. C.
KeywordsMicrobial community
On-farm biopurification system
Carendazim dissipation
Carbendazim dissipation
Issue date2013
Abstract(s)The impact of repeated carbendazim (CARB) applications on the extent of \CARB\ dissipation, the microbial diversity, the community level physiological profile (CLPP), and the enzymatic activity within the biomixture of an on-farm biopurification system was evaluated. After three successive \CARB\ applications, the \CARB\ dissipation efficiency was high; the efficiency of dissipation was 87%, 94% and 96% after each application, respectively. Although microbial enzymatic activity was affected significantly by \CARB\ application, it could recover after each \CARB\ pulse. Likewise, the numbers of cultivable bacteria, fungi and actinomycetes (as measured in CFUs) were slightly affected by the addition of CARB, but the inhibitory effect of the pesticide application was temporary. Denaturing gradient gel electrophoresis (DGGE) and Biolog Ecoplate assays demonstrated that the microbial populations remained relatively stable over time when compared to the control. The results obtained herein therefore demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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