Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/27304

TitleIdentification of glucose transporters in Aspergillus nidulans
Author(s)Reis, Thaila Fernanda dos
Menino, João F.
Pedro Bom, Vinícius Leite
Brown, Neil Andrew
Colabardini, Ana Cristina
Savoldi, Marcela
Goldman, Maria Helena S.
Rodrigues, Fernando José dos Santos
Goldman, Gustavo Henrique
Issue dateNov-2013
PublisherPLOS
JournalPLoS ONE
Abstract(s)o characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae HxtB-E were targeted to the plasma membrane. The expression of HxtB, HxtC and HxtE was able to restore growth on glucose, fructose, mannose or galactose, indicating that these transporters accept multiple sugars as a substrate through an energy dependent process. A tenfold excess of unlabeled maltose, galactose, fructose, and mannose were able to inhibit glucose uptake to different levels (50 to 80 %) in these s. cerevisiae complemented strains. Moreover, experiments with cyanide-m-chlorophenylhydrazone (CCCP), strongly suggest that hxtB, -C, and –E mediate glucose transport via active proton symport. The A. nidulans ΔhxtB, ΔhxtC or ΔhxtE null mutants showed ~2.5-fold reduction in the affinity for glucose, while ΔhxtB and -C also showed a 2-fold reduction in the capacity for glucose uptake. The ΔhxtD mutant had a 7.8-fold reduction in affinity, but a 3-fold increase in the capacity for glucose uptake. However, only the ΔhxtB mutant strain showed a detectable decreased rate of glucose consumption at low concentrations and an increased resistance to 2-deoxyglucose.
TypeArticle
URIhttp://hdl.handle.net/1822/27304
DOI10.1371/journal.pone.0081412
ISSN1932-6203
Publisher versionhttp://www.plosone.org
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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