Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/27058

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dc.contributor.authorSantos, T. C.-
dc.contributor.authorRodrigues, D. B.-
dc.contributor.authorCerqueira, M. T.-
dc.contributor.authorSilva, A. C.-
dc.contributor.authorRodrigues, A. I.-
dc.contributor.authorLeonor, I. B.-
dc.contributor.authorMarques, A. P.-
dc.contributor.authorCastro, António G.-
dc.contributor.authorReis, R. L.-
dc.date.accessioned2013-12-16T18:18:29Z-
dc.date.available2013-12-16T18:18:29Z-
dc.date.issued2013-10-
dc.date.submitted2013-10-
dc.identifier.citationSantos T. C., Rodrigues D. B., Cerqueira M. T., Silva A. C., Rodrigues A. I., Leonor I. B., Marques A. P., Castro A. G., Reis R. L. Tissue engineered constructs based on human adipose tissue derived stem cells and starch-based scaffolds for allogeneic approaches in Tissue Engineering and Regenerative Medicine, Journa of Tissue Engineering and Regenerative Medicine, doi:10.1002/term.2013.7.issue-s1, 2013-
dc.identifier.urihttp://hdl.handle.net/1822/27058-
dc.descriptionPublicado em "Journa of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)por
dc.description.abstractThe combination of natural-origin materials with mesenchymal stem cells (MSCs) from different sources have been suggested as suitable constructs for Tissue Engineering and Regenerative Medicine (TERM). Considering the importance of the host immune response to implanted tissue engineered constructs (TECs) and the immunomodulatory role assigned to MSCs and to adipose derived stem cells (ASCs) in particular, this work aimed at understanding the impact of SPCL-hASCs based TECs in the host inflammatory/immune system cells. The in vitro profile of inflammatory and anti-inflammatory cytokines expressed by macrophages (MØ) and dendritic cells (DCs), in contact with the scaffolds and the SPCL-hASCs based TECs, was evaluated by quantitative polymerase chain reaction (qRT-PCR). After 12h and 24h of direct contact, it was found that the level of expression of IL-10, IL-4, IL-6 and TNF both in MØ and DCs was bellow the detection limit. Additionally, the scaffolds and the SPCL-hASCs based TECs were intraperitoneally implanted in Balb/c mice in order to assess the expression of specific biomarkers of relevant immune cells through qRT-PCR, namely MØ, polymorphonuclear neutrophils (PMNS), B and T lymphocytes and DCs. Over the time course of the experiment comprising 7 days it was possible to verify that the SPCL scaffold seemed to trigger a less aggressive inflammatory response seen by a diminished expression of the evaluated immune cell markers when compared to the negative control. When comparing the TECs to the controls and the scaffold, TECs seemed to have the most promising results, that is, a diminished response throughout all the studied inflammatory/immune cell types was verified.<br>These findings suggest that the combination of SPCL scaffolds with hASCs offer a promise strategy for allogeneic approaches in the TERM field, also corroborating the reduced immunogenic properties of hASCs.por
dc.language.isoengpor
dc.publisherJohn Wiley and Sonspor
dc.rightsrestrictedAccesspor
dc.subjectAllogeneic Tissue Engineering & Regenerative Medicine Therapiespor
dc.subjectHuman adipose tissue derived stem cellspor
dc.subjectStarch-based constructspor
dc.titleTissue engineered constructs based on human adipose tissue derived stem cells and starch-based scaffolds for allogeneic approaches in tissue engineering and regenerative medicinepor
dc.typeconferenceAbstract-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/term.2013.7.issue-s1/issuetocpor
dc.commentshttp://www.3bs.uminho.pt/node/17731por
sdum.publicationstatuspublishedpor
oaire.citationConferenceDate10 - 12 Oct. 2013por
sdum.event.typeconferencepor
oaire.citationStartPage1por
oaire.citationEndPage2por
oaire.citationConferencePlacePorto, Portugalpor
oaire.citationTitleTERM STEM 2013por
dc.date.updated2013-11-04T10:18:29Z-
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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