Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/27005

TitleInfluence of scaffold composition over in vitro osteogenic differentiation of hBMSCs and in vivo inflammatory response
Author(s)Pinto, A. R.
Vargel, I.
Tuzlakoglu, K.
Correlo, V. M.
Sol, P. C.
Faria, Susana
Piskin, Erhan
Reis, R. L.
Neves, N. M.
KeywordsBiomaterials
Chitosan
Regenerative medicine
Scaffolds
Tissue engineering
Issue date2014
PublisherSAGE
JournalJournal of Biomaterials Applications
Abstract(s)To understand the role of chitosan in chitosan-poly(butylene succinate) scaffolds (50% wt), 50%, 25%, and 0% of chitosan were used to produce different scaffolds. These scaffolds were in vitro seeded and cultured with human bone marrow stromal cells in osteogenic conditions, revealing that higher percentage of chitosan showed enhanced cell viability over time, adhesion, proliferation, and osteogenic differentiation. Scaffolds were also implanted in cranial defects and iliac submuscular region in Wistar rats, and the results evidenced that chitosan-containing scaffolds displayed mild inflammatory response and good integration with surrounding tissues, showed by connective tissue colonization and the presence of new blood vessels. Scaffolds without chitosan-evidenced necrotic tissue in scaffolds’ interior, proving that chitosan exerts a positive effect over cell behavior and displays a milder host inflammatory response in vivo.
Typearticle
URIhttp://hdl.handle.net/1822/27005
DOI10.1177/0885328213512385
ISSN0885-3282
Peer-Reviewedyes
AccessrestrictedAccess
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
17853-J Biomater Appl-2013-Costa-Pinto-0885328213512385.pdf1,02 MBAdobe PDFView/Open    Request a copy!

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis