Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/26870

TitleCombinatorial analysis of marine based biomaterials: high-throughput analysis of the effect of nanostructured multilayers on cell behaviour
Author(s)Neto, Ana I.
Vasconcelos, Natália L.
Oliveira, Sara M.
Mano, J. F.
KeywordsHigh-throughput
Mussel adhesive
Layer-by-layer
Issue dateSep-2013
PublisherJohn Wiley and Sons
Abstract(s)In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface allowing generate irreversible bonding. Adhesive secreted proteins of mussels present an unusual amino acid 3,4-dihydroxyphenylalanine (DOPA). Inspired by the struc- ture and properties of mussel adhesive proteins, layer-by-layer (LbL) coatings based on polymers that contain catechol groups were devel- oped. We used dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry to form thin and surface-adherent dopamine films. The multilayer films were developed by electrostatic interactions using chitosan (CHT) as polycation and HA-DN as polyanion. Multilay- ers films of CHT and HA were used as control. The formation of these films was investigated in-situ by quartz crystal microbalance with dissi- pation monitoring (QCM-D). Afterwards, many combinations of the marine inspired biomaterials were analysed in a high-throughput (HTS) way. Such multilayers were constructed and individually dis- posed on isolated transparent spots, patterned onto biomimetic super- hydrophobic substrates. The adhesion properties of the coatings in the chips were also analyzed. In vitro tests using two distinct cell sources were carried out to evaluate the biological performance of the different combinations of multilayers that could be useful in different biomedical applications, including tissue engineering.
TypeAbstract
DescriptionPublicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
URIhttp://hdl.handle.net/1822/26870
ISSN1932-7005
Publisher versionhttp://onlinelibrary.wiley.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

Files in This Item:
File Description SizeFormat 
17823-abstract TermStem2013_Combinatorial analysis .pdf86,64 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID