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|Title:||Development and characterization of a PHB-HV-based 3D scaffold for a tissue engineering and cell-therapy combinatorial approach for spinal cord injury regeneration|
|Author(s):||Samy, S. M.|
Correlo, V. M.
Fraga, J. S.
Castro, Andreia Cristina Teixeira de
Almeida, Hugo Leite
Gimble, Jeffrey M.
Salgado, A. J.
Reis, R. L.
PHB-HV 3D scaffolds
Spinal Cord Injury regeneration
|Citation:||Ribeiro-Samy S. M., Silva N. A., Correlo V. M., Fraga J. S., Pinto L. A., Teixeira-Castro A., Leite-Almeida H., Almeida A., Gimble J. M., Sousa N., Salgado A. J., Reis R. L. Development and Characterization of a PHB-HV-based 3D Scaffold for a Tissue Engineering and Cell-therapy Combinatorial Approach for Spinal Cord Injury Regeneration, Macromolecular Bioscience, doi:10.1002/mabi.201300178, 2013|
|Abstract(s):||Spinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)- based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrateco- 3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence, this report addresses the development and characterization of PHB-HV-based 3D scaffolds, produced by freeze-drying, aimed to SCI treatment. The obtained scaffolds reveal an anisotropic morphology with a fully interconnected network of pores. In vitro studies demonstrate a lack of cytotoxic effect of PHB-HV scaffolds. Direct contact assays also reveal their ability to support the culture of CNS-derived cells and mesenchymal-like stem cells from different sources. Finally, histocompatibility studies show that PHB-HV scaffolds are well tolerated by the host tissue, and do not negatively impact the left hindlimb locomotor function recovery. Therefore results herein presented suggest that PHB-HV scaffolds may be suitable for SCI treatment.|
|Access:||Restricted access (UMinho)|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|
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|17662-Development and Characterization of a PHB-HV-based 3D Scaffold for a Tissue Engineering and Cell-Therapy combinatorial approach for Spinal Cord Injury Regeneration.pdf|
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