Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/26836

TitleDevelopment and characterization of a PHB-HV-based 3D scaffold for a tissue engineering and cell-therapy combinatorial approach for spinal cord injury regeneration
Author(s)Samy, S. M.
Silva, Nuno
Correlo, V. M.
Fraga, J. S.
Pinto, Luísa
Castro, Andreia Cristina Teixeira de
Almeida, Hugo Leite
Almeida, Armando
Gimble, Jeffrey M.
Sousa, Nuno
Salgado, A. J.
Reis, R. L.
KeywordsBiocompatibility
Cell-based therapy
PHB-HV 3D scaffolds
Spinal Cord Injury regeneration
Tissue engineering
Issue dateAug-2013
PublisherWiley
JournalMacromolecular Bioscience
CitationRibeiro-Samy S. M., Silva N. A., Correlo V. M., Fraga J. S., Pinto L. A., Teixeira-Castro A., Leite-Almeida H., Almeida A., Gimble J. M., Sousa N., Salgado A. J., Reis R. L. Development and Characterization of a PHB-HV-based 3D Scaffold for a Tissue Engineering and Cell-therapy Combinatorial Approach for Spinal Cord Injury Regeneration, Macromolecular Bioscience, doi:10.1002/mabi.201300178, 2013
Abstract(s)Spinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)- based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrateco- 3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence, this report addresses the development and characterization of PHB-HV-based 3D scaffolds, produced by freeze-drying, aimed to SCI treatment. The obtained scaffolds reveal an anisotropic morphology with a fully interconnected network of pores. In vitro studies demonstrate a lack of cytotoxic effect of PHB-HV scaffolds. Direct contact assays also reveal their ability to support the culture of CNS-derived cells and mesenchymal-like stem cells from different sources. Finally, histocompatibility studies show that PHB-HV scaffolds are well tolerated by the host tissue, and do not negatively impact the left hindlimb locomotor function recovery. Therefore results herein presented suggest that PHB-HV scaffolds may be suitable for SCI treatment.
TypeArticle
URIhttp://hdl.handle.net/1822/26836
DOI10.1002/mabi.201300178/pdf
ISSN1616-5195
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/mabi.201300178/pdf
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals


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