Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/26834

TitleDesign and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
Author(s)Requicha, J. F.
Viegas, C. A. A.
Hede, Shantesh
Leonor, I. B.
Reis, R. L.
Gomes, Manuela E.
KeywordsBiodegradable
Guided tissue regeneration
Osteoconductive groups
Periodontium
Scaffold
Tissue engineering
Issue date2016
PublisherWiley
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationRequicha, J. F., Viegas, C. A., Hede, S., Leonor, I. B., Reis, R. L., & Gomes, M. E. (2016). Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications. Journal of Tissue Engineering and Regenerative Medicine, 10(5), 392-403. doi: 10.1002/term.1816
Abstract(s)The inefficacy of the currently used therapies in achieving the regeneration ad integrum of the periodontium stimulates the search for alternative approaches, such as tissue-engineering strategies. Therefore, the core objective of this study was to develop a biodegradable double-layer scaffold for periodontal tissue engineering. The design philosophy was based on a double-layered construct obtained from a blend of starch and poly-ε-caprolactone (30:70 wt%; SPCL). A SPCL fibre mesh functionalized with silanol groups to promote osteogenesis was combined with a SPCL solvent casting membrane aiming at acting as a barrier against the migration of gingival epithelium into the periodontal defect. Each layer of the double-layer scaffolds was characterized in terms of morphology, surface chemical composition, degradation behaviour and mechanical properties. Moreover, the behaviour of seeded/cultured canine adipose-derived stem cells (cASCs) was assessed. In general, the developed double-layered scaffolds demonstrated adequate degradation and mechanical behaviour for the target application. Furthermore, the biological assays revealed that both layers of the scaffold allow adhesion and proliferation of the seeded undifferentiated cASCs, and the incorporation of silanol groups into the fibre-mesh layer enhance the expression of a typical osteogenic marker. This study allowed an innovative construct to be developed, combining a three-dimensional (3D) scaffold with osteoconductive properties and with potential to assist periodontal regeneration, carrying new possible solutions to current clinical needs .
TypeArticle
DescriptionFirst published: 1 September 2013
URIhttp://hdl.handle.net/1822/26834
DOI10.1002/term.1816
ISSN1932-7005
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/term.1816/abstract
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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