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|Title:||A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths|
|Author(s):||Correia, Tiago R.|
Antunes, Bernardo P.
Castilho, Pedro H.
Nunes, José C.
Amorim, M. T. Pessoa de
Escobar, I. C.
Correia, I. J.
|Journal:||Separation and Purification Technology|
|Abstract(s):||The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.|
|Appears in Collections:||DET/2C2T - Artigos em revistas internacionais com arbitragem científica|
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