Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/26707

TitleA bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths
Author(s)Correia, Tiago R.
Antunes, Bernardo P.
Castilho, Pedro H.
Nunes, José C.
Amorim, M. T. Pessoa de
Escobar, I. C.
Correia, I. J.
Morão, A.
KeywordsMicrofiltration
Electrospinning
Bi-layer membrane
Lysate
Plasmid DNA
DNA
Plasmid
Issue date2013
PublisherElsevier
JournalSeparation and Purification Technology
Abstract(s)The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.
TypeArticle
URIhttp://hdl.handle.net/1822/26707
DOI10.1016/j.seppur.2013.03.049
ISSN1383-5866
Publisher versionhttp://dx.doi.org/10.1016/j.seppur.2013.03.049
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

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