Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/2655

TitleTreatment of wool fibres with subtilisin and subtilisin-PEG
Author(s)Silva, Carla J. S. M.
Prabaharan, M.
Gübitz, Georg M.
Paulo, Artur Cavaco
KeywordsSerine proteases
Protein adsorption
Wool fibre
Clean technology
Cross linking
wool fibres clean technology
Issue date2005
PublisherElsevier
JournalEnzyme and microbial technology
Citation"Enzyme and microbial technology". ISSN 0141-0229. 36:7 (2005) 917-922.
Abstract(s)In this work the diffusion of serine proteases into wool fabrics and yarns was studied. The proteases used were free subtilisin and subtilisin-PEG (the same enzyme that was covalently cross linked to polyethylene glycol). It is shown that the adsorption and diffusion is facilitated by the pre-treatment performed, being the alkaline surfactant washing and bleaching the most effective in what concerns enzyme adsorption. Furthermore, this study suggests that the diffusion of proteases into wool is dependent on the size of the protease. The free enzyme penetrates into wool fibre cortex while the modified bigger enzyme is retained only at the surface, in the cuticle layer. Also, proteins without proteolytic activity do not adsorb considerably on wool due to its hydrophobic nature, therefore the diffusion is facilitated by hydrolytic action. These results have important practical implications for the establishment of enzymatic wool finishing processes, since they allow for control of the enzyme hydrolysis, which was the major drawback of this environmental friendly option to the conventional chlorine treatments.
TypeArticle
URIhttp://hdl.handle.net/1822/2655
DOI10.1016/j.enzmictec.2005.01.017
ISSN0141-0229
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

Files in This Item:
File Description SizeFormat 
72[1].pdf307,73 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID