Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/26104

TitleHydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacillus megaterium BM3
Author(s)Hasmann, Andrea
Eberl, Anita
Schneider, Konstantin
Schoening, K.-U.
Kaufmann, Franz
Glieder, Anton
Kovac, Janez
Paulo, Artur Cavaco
Wehrschuetz-Sigl, Eva
Guebitz, G. M.
KeywordsOxidoreductase
Polypropylene
Polymer
Surface modification
Issue date2012
PublisherInforma Healthcare
JournalBiocatalysis and Biotransformation
Abstract(s)Enzymatic hydroxylation of polypropylene (PP) was investigated in order to increase hydrophilicity. A mutant (139-3) of the P450monooxygenase from Bacillus megaterium expressed in E. coli DH5α was purified using anion exchange chromatography. Hydroxylation of PP fabrics led to a dramatic increase of hydrophilicity as indicated by a water drop dissipation time of below 1 s compared to the hydrophobic reference material. Likewise, a 4.9 cm increase of rising height was measured which remained consistent after 144 h of storage. Similarly, enzymatic hydroxylation of PP films lead to a decrease of the WCA from 104.6° to 77.3° with no major change after exposure to air for 6 days. Using X-ray photoelectron spectroscopy, an increase in normalized atomic concentrations of oxygen from 1.40 to 4.98% for the CO-inhibited and enzyme treated sample, respectively, was measured confirming enzymatic hydroxylation.
TypeArticle
URIhttp://hdl.handle.net/1822/26104
DOI10.3109/10242422.2012.645367
ISSN1024-2422
1029-2446
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica


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