Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/26085

TitleEncapsulation of RNA molecules in BSA microspheres and internalization into Trypanosoma Brucei parasites and human U2OS cancer cells
Author(s)Shimanovich, Ulyana
Tkacz, Itai Dov
Eliaz, Dror
Paulo, Artur Cavaco
Michaeli, S.
Gedanken, Aharon
Keywordsdrug delivery
microspheres
RNA
somochemistry
Issue date2011
PublisherWiley
JournalAdvanced Functional Materials
Abstract(s)RNA was encapsulated in bovine serum albumin (BSA) microspheres using a one-step sonochemical process from an water–oil solvent biphasic system. Confocal microcoscopy and fluorescence-activated cell sorting indicate that a CY3-RNA (RNA labeled with red fluorescent indocarbocyanine Cy3 dye) sphere is encapsulated in the BSA outer sphere. The diameter of the sphere depends on the number of nucleotides of the RNA, ranging from 0.63 to 2.74 μm. Total RNA (t-RNA) was used as a prototype for the future small interfering RNA (siRNA) delivery. A very broad size distribution characterizes the RNA spheres and therefore, among the loaded BSA spheres, there were sufficiently small spheres to be successfully introduced into trypanosoma brucei parasites and human osteosarcoma U2OS cancer cells.
TypeArticle
URIhttp://hdl.handle.net/1822/26085
DOI10.1002/adfm.201100963
ISSN1616-3028
Publisher versionDOI: 10.1002/adfm.201100963
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

Files in This Item:
File Description SizeFormat 
2011 Encapsulation of RNA Molecules in BSA Microspheres and Internalization into Trypanosoma Brucei Parasites and Human U2OS Cancer Cells.pdfEncapsulation of RNA Molecules in BSA Microspheres and Internalization into Trypanosoma Brucei Parasites and Human U2OS Cancer Cells2,14 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID