Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/2574
Título: | Use of two different carriers in a packed bed reactor for endopolygalacturonase production by a yeast strain |
Autor(es): | Almeida, Catarina Brányik, Tomáš Ferreira, Pedro Moradas Teixeira, J. A. |
Palavras-chave: | Continuous reactor Immobilized yeast cells Kluyveromyces marxianus Pectinase Siran Spent grains |
Data: | Abr-2005 |
Editora: | Elsevier |
Revista: | Process biochemistry |
Citação: | "Process biochemistry". ISSN 1359-5113. 40:5 (2005) 1937-1942. |
Resumo(s): | A packed bed reactor (PBR) design was tested for the purpose of continuous pectinase production with yeasts, as a possible alternative to the traditional batch process using fungal cultures. Two different carriers - a porous glass (Siran) and a cellulosic carrier obtained from spent grains (barley) - were used to immobilize Kluyveromyces marxianus CCT 3172, a yeast strain secreting endopolygalacturonase. To improve cell distribution throughout the column, part of the outflow was recycled. Cell loads of 0.204 and 0.247 g(biomass)/g(carrier) were obtained at the top and bottom of the PBR with spent grains, respectively. Using the PBR with Siran as the immobilization support, 0.071 g(biomass)/g(carrier) was the biomass load at the top of the column while at the bottom a value of 0.147 g(biomass)/g(carrier) was found. The highest value for pectinase volumetric productivity (Pv = 1.68 U/ml h) was achieved in the PBR with Siran for a D = 0.260 h(-1) and a glucose concentration on the inlet of S-in = 40 g/l. Both carriers were suitable for pectinase production. The best results were obtained with a high and uniform biomass concentration in the column, together with high dilution rates and total glucose consumption. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/2574 |
DOI: | 10.1016/j.procbio.2004.07.008 |
ISSN: | 1359-5113 |
Versão da editora: | http://www.elsevier.com/wps/find/homepage.cws_home http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6THB-4DFBVV5-3-1&_cdi=5278&_user=2459786&_orig=search&_coverDate=04%2F01%2F2005&_sk=999599994&view=c&wchp=dGLzVzz-zSkWW&md5=446fd2aaabb0c96f67616cd408526c75&ie=/sdarticle.pdf |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ProcessBiochemistry-Almeida[1].pdf | 260,9 kB | Adobe PDF | Ver/Abrir |