Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/25429

TitleSilk hydrogels from non-mulberry and mulberry silkworm cocoons processed with ionic liquids
Author(s)Silva, Simone Santos
Popa, Elena Geta
Gomes, Manuela E.
Oliveira, Mariana B.
Nayak, S.
Bano, S.
Mano, J. F.
Kundu, S. C.
Reis, R. L.
Keywords3D scaffolds
Ionic liquids
Silk fibroin
Bombyx mori
Fibroin
Antheraea mylitta
Hydrogels
Issue dateJul-2013
PublisherElsevier
JournalActa Biomaterialia
Abstract(s)Matrices based on silk fibroin from the non-mulberry silkworm Antheraea mylitta and the mulberry silkworm Bombyx mori have demonstrated good applicability in regenerative medicine. However, the cocoons of A. mylitta are underutilized in part due to their lack of solubility in traditional organic solvents. Therefore, the present work investigates the solubilization and processing of degummed fibers obtained from the cocoons of both silkworm species into hydrogels using ionic liquids (ILs). The developed hydrogels exhibited a rubbery consistency, viscoelastic behavior and rapid degradation in the presence of protease XIV. Scanning electron and confocal microscopy images suggest that human adipose stem cells (hASCs) are able to adhere to and migrate at different levels within the hydrogel structures. Moreover, the MTS assay demonstrated the maintenance of cell metabolic activity for up to 28days, while DNA quantification showed that hASCs were able to proliferate on the seeded hydrogels. The findings indicate that complete IL removal from the fabricated hydrogels results in a positive hASCs cellular response. Thus the present approach provides a unique opportunity to broaden the processability and application of silk fibroin obtained from A. mylitta cocoons for regenerative medicine, namely cartilage regeneration.
TypeArticle
URIhttp://hdl.handle.net/1822/25429
DOI10.1016/j.actbio.2013.06.044
ISSN1742-7061
Publisher versionhttp://www.sciencedirect.com/science/article/pii/S1742706113003371
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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