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|Title:||Development and characterization of PHB-HV based 3D scaffolds for a tissue engineering and cell-therapy combinatorial approach for spinal cord Injury regeneration|
|Author(s):||Samy, S. M.|
Silva, Nuno A.
Correlo, Vitor M.
Fraga, Joana S.
Gimble, Jeffrey M.
Salgado, António J.
Reis, R. L.
PHB-HV 3D scaffolds
spinal cord injury regeneration
|Abstract(s):||Spinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)- based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrateco- 3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence, this report addresses the development and characterization of PHB-HV-based 3D scaffolds, produced by freeze-drying, aimed to SCI treatment. The obtained scaffolds reveal an anisotropic morphology with a fully interconnected network of pores. In vitro studies demonstrate a lack of cytotoxic effect of PHB-HV scaffolds. Direct contact assays also reveal their ability to support the culture of CNS-derived cells and mesenchymal-like stem cells from different sources. Finally, histocompatibility studies show that PHB-HV scaffolds are well tolerated by the host tissue, and do not negatively impact the left hindlimb locomotor function recovery. Therefore results herein presented suggest that PHB-HV scaffolds may be suitable for SCI treatment.|
|Appears in Collections:||ICVS - Artigos em Revistas Internacionais com Referee|
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|ribeiro-samy s_macrom biosc 2013 posp.pdf||1,73 MB||Adobe PDF||View/Open|