Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/25171

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dc.contributor.authorFerreira, D. S.-
dc.contributor.authorReis, R. L.-
dc.contributor.authorAzevedo, Helena S.-
dc.date.accessioned2013-09-17T10:35:46Z-
dc.date.available2013-09-17T10:35:46Z-
dc.date.issued2013-10-
dc.date.submitted2013-09-
dc.identifier.issn1744-683Xpor
dc.identifier.urihttp://hdl.handle.net/1822/25171-
dc.description.abstractPeptides are excellent building blocks to form precise nanostructures by self-assembly. They can selfassemble into fibril nanostructures, thus recreating some of the architectural features of the natural extracellular matrix. Here, we used a microfluidic approach to drive the self-assembly of peptides of opposite charge into capsular structures for cell encapsulation. The obtained capsules presented a core shell structure made of a network of nanofibers and their properties can be tuned by varying the concentration of each peptide. Capsules were found to be stable in aqueous solutions and their permeability dependent on the capsule composition. Human dermal fibroblasts were encapsulated and remained viable within the capsules and their morphology was shown to be influenced by the matrix density. Additionally, these capsules also supported the co-culture of fibroblasts and keratinocytes. We expect that the developed peptide-based microcapsules can serve as miniaturized environments for cell culture and as biomimetic platforms for in vitro drug screening.por
dc.description.sponsorshipFunding for this study was provided by the Portuguese Foundation for Science and Technology (FCT, grant PTDC/EBB-BIO/114523/2009). D. S. Ferreira gratefully acknowledges FCT for the PhD scholarship (SFRH/BD/44977/2008). We also thank Dr A. C. Mendes from the 3B's Research Group at the University of Minho (Portugal) for her assistance with the microfluidics device.por
dc.language.isoengpor
dc.publisherRoyal Society of Chemistrypor
dc.rightsrestrictedAccesspor
dc.subjectCell culturepor
dc.subjectDirected self-assemblypor
dc.subjectElectrostatic self-assemblypor
dc.subjectMicrofluidicspor
dc.subjectPeptide-amphiphilespor
dc.subjectSoft capsulespor
dc.titlePeptide-based microcapsules obtained by self-assembly and microfluidics as controlled environments for cell cultureeng
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://pubs.rsc.org/en/content/articlelanding/2013/sm/c3sm51189h#!divAbstractpor
dc.commentshttp://www.3bs.uminho.pt/node/17658por
sdum.publicationstatuspublishedpor
degois.publication.firstPage9237por
degois.publication.lastPage9248por
degois.publication.issue38por
degois.publication.titleSoft Matterpor
degois.publication.volume9por
dc.date.updated2013-09-12T09:03:17Z-
dc.identifier.doi10.1039/c3sm51189hpor
dc.subject.wosScience & Technologypor
sdum.journalSoft Matterpor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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