Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/25167

TitlePorous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
Author(s)Silva, S. P.
Moreira-Silva, J.
Silva, Tiago H.
Perez-Martin, R. I.
Sotelo, C. G.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
KeywordsCollagen
Hydrogels
Marine biotechnolgoy
Supercritical fluids
Tissue engineering
marine biotechnology
Issue dateSep-2013
PublisherWiley
JournalMacromolecular Bioscience
Abstract(s)The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare prescaffolds by freeze-drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO2 atmosphere, crosslinking of collagen pre-scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte-like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering.
TypeArticle
URIhttp://hdl.handle.net/1822/25167
DOI10.1002/mabi.201300228
ISSN1616-5195
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/mabi.201300228/abstract
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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