Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24934

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dc.contributor.authorAlmeida, Carina-
dc.contributor.authorAzevedo, N. F.-
dc.contributor.authorBento, Joana C.-
dc.contributor.authorCerca, Nuno-
dc.contributor.authorRamos, H.-
dc.contributor.authorVieira, M. J.-
dc.contributor.authorKeevil, C. W.-
dc.date.accessioned2013-08-05T16:20:16Z-
dc.date.available2013-08-05T16:20:16Z-
dc.date.issued2013-
dc.identifier.issn0934-9723por
dc.identifier.issn1435-4373por
dc.identifier.urihttp://hdl.handle.net/1822/24934-
dc.description.abstractWe developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.por
dc.description.sponsorshipWe would like to thank Cristina Silva for providing us the urine samples. This work was supported by Fundacao para a Ciencia e Tecnologia (Postdoctoral Fellowship SFRH/BPD/74480/2010 and project PIC/IC/82815/2007) and by the European Commission's Framework Programme (FP7: "COST-Action TD1004: Theragnostics for imaging and therapy").por
dc.language.isoengpor
dc.publisherSpringerpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5646-ICCMS/82815/PT-
dc.rightsopenAccesspor
dc.titleRapid detection of urinary tract infections caused by Proteus spp. using PNA-FISHpor
dc.typearticlepor
dc.peerreviewedyespor
sdum.publicationstatuspublishedpor
oaire.citationStartPage781por
oaire.citationEndPage786por
oaire.citationIssue6por
oaire.citationTitleEuropean Journal of Clinical Microbiology and Infectious Diseasespor
oaire.citationVolume32por
dc.publisher.uriSpringer Verlagpor
dc.identifier.essn1435-4373por
dc.identifier.doi10.1007/s10096-012-1808-2por
dc.identifier.pmid23288291por
dc.subject.wosScience & Technologypor
sdum.journalEuropean Journal of Clinical Microbiology and Infectious Diseasespor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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