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TitleRapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
Author(s)Almeida, Carina
Azevedo, N. F.
Bento, Joana C.
Cerca, Nuno
Ramos, H.
Vieira, M. J.
Keevil, C. W.
Issue date2013
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Abstract(s)We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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