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|Title:||Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH|
Azevedo, N. F.
Bento, Joana C.
Vieira, M. J.
Keevil, C. W.
|Journal:||European Journal of Clinical Microbiology and Infectious Diseases|
|Abstract(s):||We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|