Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24925

TitleLaccase production by free and immobilized mycelia of Peniophora cinerea and Trametes versicolor: a comparative study
Author(s)Silvério, Sara C.
Moreira, Sérgio
Milagres, Adriane M. F.
Macedo, Eugénia A.
Teixeira, J. A.
Mussatto, Solange I.
KeywordsLaccase
Fermentation
Immobilisation
Peniophora cinerea
Trametes versicolor
Bioreactor
Issue date2013
PublisherSpringer
JournalBioprocess and Biosystems Engineering
Abstract(s)The production of laccase by immobilized mycelia of Peniophora cinerea and Trametes versicolor was studied. In an initial stage, experimental assays were performed in Erlenmeyer flasks using free and immobilized mycelium, and the performance of the fungal strains to produce the enzyme was compared. Both fungi adhered into the support material (a synthetic fiber), growing not only on the surface but also in the interspaces of the fibers. Immobilization of P. cinerea provided a 35-fold increase in laccase production when compared to the production obtained by using free mycelium. On the other hand, immobilization of T. versicolor caused a decrease in laccase activity. A comparison between the strains revealed that immobilized P. cinerea (3,500 U/L) surpassed the enzyme production by free T. versicolor (800 U/L). When the conditions that gave the best laccase production to each fungus were employed in a stirred tank bioreactor, very low laccase production was observed for both the cases, suggesting that shear stress and mycelia damage caused by the agitation impellers negatively affected the enzyme production.
TypeArticle
URIhttp://hdl.handle.net/1822/24925
DOI10.1007/s00449-012-0793-2
ISSN1615-7591
1615-7605
e-ISSN1615-7605
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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