Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24905

TitleSecondary structure of rhBMP-2 in a protective biopolymeric carrier material
Author(s)Gilde, Flora
Maniti, Ofélia
Guillot, Raphael
Mano, J. F.
Logeart-Avramoglou, Delphine
Sailhan, Frédéric
Picart, Catherine
KeywordsATR-FTIR
Differentiation
Growth-factors
Inhibition
Microenvironments
One morphogenetic protein-2
Polyelectrolyte multilayer films
Stability
Tissue
BMP-2
Issue date2012
PublisherAmerican Chemical Society (ACS)
JournalBiomacromolecules
Abstract(s)Efficient delivery of growth factors is one of the great challenges of tissue engineering. Polyelectrolyte multilayer films (PEM) made of biopolymers have recently emerged as an interesting carrier for delivering recombinant human bone morphogenetic protein 2 (rhBMP-2 noted here BMP-2) to cells in a matrix-bound manner. We recently showed that PEM made of poly(l-lysine) and hyaluronan (PLL/HA) can retain high and tunable quantities of BMP-2 and can deliver it to cells to induce their differentiation in osteoblasts. Here, we investigate quantitatively by Fourier transform infrared spectroscopy (FTIR) the secondary structure of BMP-2 in solution as well as trapped in a biopolymeric thin film. We reveal that the major structural elements of BMP-2 in solution are intramolecular β-sheets and unordered structures as well as α-helices. Furthermore, we studied the secondary structure of rhBMP-2 trapped in hydrated films and in dry films since drying is an important step for future applications of these bioactive films onto orthopedic biomaterials. We demonstrate that the structural elements were preserved when BMP-2 was trapped in the biopolymeric film in hydrated conditions and, to a lesser extent, in dry state. Importantly, its bioactivity was maintained after drying of the film. Our results appear highly promising for future applications of these films as coatings of biomedical materials, to deliver bioactive proteins while preserving their bioactivity upon storage in dry state.
TypeArticle
URIhttp://hdl.handle.net/1822/24905
DOI10.1021/bm3010808
ISSN1525-7797
Publisher versionhttp://dx.doi.org/10.1021/bm3010808
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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