Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/24885

TítuloPHD2 regulates arteriogenic macrophages through TIE2 signaling
Autor(es)Hamm, Alexander
Veschini, Lorenzo
Takeda, Yukiji
Costa, Sandra Maria Araújo da
Delamarre, Estelle
Squadrito, Mario Leonardo
Henze, Anne-Theres
Wenes, Mathias
Serneels, Jens
Pucci, Ferdinando
Roncal, Carmen
Anisimov, Andrey
Alitalo, Kari
De Palma, Michele
Mazzone, Massimiliano
EditoraJohn Wiley and Sons
RevistaEmbo Molecular Medicine
Resumo(s)Occlusion of the main arterial route redirects blood flow to the collateral circulation. We previously reported that macrophages genetically modified to express low levels of prolyl hydroxylase domain protein 2 (PHD2) display an arteriogenic phenotype, which promotes the formation of collateral vessels and protects the skeletal muscle from ischaemic necrosis. However, the molecular mechanisms underlying this process are unknown. Here, we demonstrate that femoral artery occlusion induces a switch in macrophage phenotype through angiopoietin-1 (ANG1)-mediated Phd2 repression. ANG blockade by a soluble trap prevented the downregulation of Phd2 expression in macrophages and their phenotypic switch, thus inhibiting collateral growth. ANG1-dependent Phd2 repression initiated a feed-forward loop mediated by the induction of the ANG receptor TIE2 in macrophages. Gene silencing and cell depletion strategies demonstrate that TIE2 induction in macrophages is required to promote their proarteriogenic functions, enabling collateral vessel formation following arterial obstruction. These results indicate an indispensable role for TIE2 in sustaining in situ programming of macrophages to a proarteriogenic, M2-like phenotype, suggesting possible new venues for the treatment of ischaemic disorders.
Versão da editorawww.onlinelibrary.wiley.com
Arbitragem científicayes
Aparece nas coleções:ICVS - Artigos em Revistas Internacionais com Referee

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