Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24880

TitleVascular Endothelial Growth Factor and Fibroblast Growth Factor-2 Incorporation in Starch-Based Bone Tissue-Engineered Constructs Promote the In Vivo Expression of Neovascularization Mediators
Author(s)Santos, T. C.
Morton, Tatjana J.
Moritz, Martina
Pfeifer, Sabine
Reise, Kathrin
Marques, A. P.
Castro, António G.
Reis, R. L.
Griensven, Martijn van
KeywordsFGF-2
Tissue-engineered constructs
Vascularization
VEGF
Issue dateApr-2013
PublisherMary Ann Liebert Inc.
JournalTissue Engineering, Part A
Abstract(s)The ideal bone tissue-engineered (TE) construct remains to be found, although daily discoveries significantly contribute to improvements in the field and certainly have valuable long-term outcomes. In this work, different TE elements, aiming at bone TE applications, were assembled and its effect on the expression of several vas- cularization/angiogenesis mediators analyzed. Starch/polycaprolactone (SPCL) scaffolds, obtained by two different methodologies, were combined with fibrin sealant (Baxter), human adipose-derived stem cells (hASCs), and growth factors (vascular endothelial growth factor [VEGF] or fibroblast growth factor-2 [FGF-2]), and implanted in vascular endothelial growth factor receptor-2 (VEGFR2)-luc transgenic mice. The expression of VEGFR2 along the implantation of the designed constructs was followed using a luminescence device (XenogenÒ) and after 2 weeks, the explants were retrieved to perform histological analysis and reverse transcriptase–polymerase chain reaction for vascularization (VEGF and VEGFR1) and inflammatory (tumor necrosis factor-alpha, interleukin-4, and interferon-gamma) markers. It was showed that SPCL scaffolds ob- tained by wet spinning and by fiber bonding constitute an adequate support for hASCs. The assembled TE constructs composed by fibrin sealant, hASCs, VEGF, and FGF-2 induce only a mild inflammatory reaction after 2 weeks of implantation. Additionally, the release of VEGF and FGF-2 from the constructs enhanced the ex- pression of VEGFR2 and other important mediators in neovascularization (VEGF and VEGFR1). These results indicate the potential of VEGF or FGF-2 within a bone TE construct composed by wet-spun SPCL, fibrin sealant, and hASCs in promoting the vascularization of newly formed tissue.
TypeArticle
URIhttp://hdl.handle.net/1822/24880
DOI10.1089/ten.tea.2010.0741
ISSN2152-4947
2152-4955
Publisher versionhttp://online.liebertpub.com/doi/abs/10.1089/ten.tea.2010.0741
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals


Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID