Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/2428

TitleMetabolic flux response to salt induced stress in the halotolerant yeast Debaryomyces hansenii
Author(s)Neves, Maria Luísa Vieira das
Oliveira, Rui Pedro Soares de
Lucas, Cândida
KeywordsDebaryomyces hansenii
Halotolerance
Metabolic flux
Growth parameters
Enzyme sensitivity
Issue date1997
PublisherSociety for General Microbiology
JournalMicrobiology
Citation"Microbiology". ISSN 1350-0872. 143:4 (1997) 1133-1139.
Abstract(s)The toxic effect of NaCl and KCl, on growth of the marine yeast Debaryomyces hansenii on glucose or glycerol was studied. Above threshold value both salts inhibited specific growth rate, specific glucose and glycerol respiration rates and specific glucose fermentation rate, as well as biomass yields. The exponential inhibition constant, k, and minimum toxic concentration, cmin, were similar for all physiological parameters assayed. The effect of either salt in the specific activity of several glycolytic enzymes showed a similar inhibition pattern, although at much lower salt concentrations. In agreement with published results on glycerol phosphate dehydrogenase stimulation by salt, we present evidence in which a general glycolytic flux deviation could occur naturally during salt stress, due to the intrinsic sensitivity of the glycolytic enzymes responsible for the prosecution of glycolysis to intracellular ion concentrations.
TypeArticle
URIhttp://hdl.handle.net/1822/2428
ISSN1350-0872
1465-2080
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DBio - Artigos/Papers

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