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|Title:||Chitosan membranes for spatially controlled cell adhesion and specific cell recruitment|
|Author(s):||Custódio, Catarina A.|
Frias, A. M.
Campo, Aranzazu del
Reis, R. L.
Mano, J. F.
|Journal:||Journal of Tissue Engineering and Regenerative Medicine|
|Citation:||Custódio C. A., Frias A. M., del Campo A., Reis R. L., Mano J. F. Chitosan membranes for spatially controlled cell adhesion and specific cell recruitment, 3rd TERMIS World Congress 2012, 2012|
|Abstract(s):||We propose a concept of biomaterials that are able to fix specific cell types onto their surface when in contact with a mix population of cells. Adipose tissue has shown to be an interesting source of stem cells with therapeutic potential. However only a small amount of the heteroge- neous mixture of the cells extracted from lipoaspirates are stem cells, and within stem cells there are different populations with different capabilities to differentiate through a lineage. We studied the ability of immobilized antibodies on chitosan surfaces to capture specific types of cells with a spatial micrometer resolution.Antibodies were covalently immobilized onto chitosan membranes using bis[sulfosuccinimidyl] su- berate (BS3). X-ray photoelectron spectroscopy (XPS) was used to chemically characterize the surface and quartz crystal microbalance (QCM) to calculate the amount of adsorbed and/or immobilized anti- body. Data shown greater immobilization when BS3 was used com- pared to simple adsorption. Specific antibodies covalently immobilized in a surface, kept their bioactivity and controlled the type of cell that attached on the chitosan surface. Microcontact printing permitted to covalently immobilize antibodies in patterns allowing a spatial control in cell attachment. Cell sorting experiments performed using a mixture of adipose stem cells and osteoblast like cells shown that chitosan sur- faces were able to capture a specific phenotype depending on the immobilized antibody.|
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