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TitleChitosan membranes for spatially controlled cell adhesion and specific cell recruitment
Author(s)Custódio, Catarina A.
Frias, A. M.
Campo, Aranzazu del
Reis, R. L.
Mano, J. F.
Surface modification
Issue dateOct-2012
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationCustódio C. A., Frias A. M., del Campo A., Reis R. L., Mano J. F. Chitosan membranes for spatially controlled cell adhesion and specific cell recruitment, 3rd TERMIS World Congress 2012, 2012
Abstract(s)We propose a concept of biomaterials that are able to fix specific cell types onto their surface when in contact with a mix population of cells. Adipose tissue has shown to be an interesting source of stem cells with therapeutic potential. However only a small amount of the heteroge- neous mixture of the cells extracted from lipoaspirates are stem cells, and within stem cells there are different populations with different capabilities to differentiate through a lineage. We studied the ability of immobilized antibodies on chitosan surfaces to capture specific types of cells with a spatial micrometer resolution.Antibodies were covalently immobilized onto chitosan membranes using bis[sulfosuccinimidyl] su- berate (BS3). X-ray photoelectron spectroscopy (XPS) was used to chemically characterize the surface and quartz crystal microbalance (QCM) to calculate the amount of adsorbed and/or immobilized anti- body. Data shown greater immobilization when BS3 was used com- pared to simple adsorption. Specific antibodies covalently immobilized in a surface, kept their bioactivity and controlled the type of cell that attached on the chitosan surface. Microcontact printing permitted to covalently immobilize antibodies in patterns allowing a spatial control in cell attachment. Cell sorting experiments performed using a mixture of adipose stem cells and osteoblast like cells shown that chitosan sur- faces were able to capture a specific phenotype depending on the immobilized antibody.
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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