Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/2397

TitleNitrile hydratase and amidase from rhodococcus rhodochrous hydrolyze acrylic fibers and granular polyacrylonitriles
Author(s)Tauber, Michael
Paulo, Artur Cavaco
Robra, K. B.
Gübitz, Georg M.
Issue date2000
PublisherAmerican Society for Microbiology (ASM)
JournalApplied and Environmental Microbiology
Citation"Applied and environmental microbiology". ISSN 0099-2240. 66:4 (Apr. 2000) 1634–1638.
Abstract(s)Rhodococcus rhodochrous NCIMB 11216 produced nitrile hydratase (320 nkat mg of protein(-1)) and amidase activity (38.4 nkat mg of protein(-1)) when grown on a medium containing propionitrile. These enzymes sere able to hydrolize nitrile groups of both granular polyacrylonitriles (PAN) and acrylic fibers, Nitrile groups of PAN40 (molecular mass, 40 kDa) and PAN190 (molecular mass, 190 kDa) were converted into the corresponding carbonic acids to 1.8 and 1.0%, respectively. In contrast, surfacial nitrile groups of acrylic fibers were only converted to the corresponding amides. X-ray photoelectron spectroscopy analysis showed that 16% of the surfacial nitrile groups were hydrolyzed by the R. rhodochrous enzymes, Due to the enzymatic modification, the acrylic fibers became more hydrophilic and thus, adsorption of dyes was enhanced, This was indicated by a 15% increase in the staining level (K/S value) for C.I. Basic Blue 9.
TypeArticle
URIhttp://hdl.handle.net/1822/2397
DOI10.1128/AEM.66.4.1634-1638.2000
ISSN0099-2240
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

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