Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/23906

TitleHuman serum is a suitable supplement for the osteogenic differentiation of human adipose-derived stem cells seeded on poly-3-hydroxibutyrate-co-3-hydroxyvalerate scaffolds.
Author(s)Paula, A. C. de
Zonari, A. A. C.
Martins, T. M.
Novikoff, S.
Silva, A. R. da
Correlo, V. M.
Reis, R. L.
Gomes, D. A.
Goes, A. M.
KeywordsMedium supplementation
Osteogenic differentiation
Issue dateJan-2013
PublisherMary Ann Liebert Inc.
JournalTissue Engineering : Part A
Abstract(s)Human adipose-­‐derived stem cells (hASCs) are currently a point of focus for bone tissue engineering applications. However, the ex vivo expansion of stem cells before clinical application remains a challenge. Fetal bovine serum (FBS) is largely used as a medium supplement and exposes the recipient to infections and immunological reactions. In this study, we evaluated the osteogenic differentiation process of hASCs in poly-­‐3-­‐hydroxybutyrate-­‐co-­‐3-­‐ hydroxyvalerate (PHB-­‐HV) scaffolds with the osteogenic medium supplemented with pooled allogeneic human serum (aHS). The hASCs grown in the presence of FBS or aHS did not show remarkable differences in morphology or immunophenotype. The PHB-­‐HV scaffolds, which were developed by the freeze-­‐ drying technique, showed an adequate porous structure and mechanical performance as observed by micro-­‐computed tomography, scanning electron microscopy (SEM), and compression test. The three-­‐dimensional structure was suitable for allowing cell colonization, which was revealed by SEM micrographs. Moreover, these scaffolds were not toxic to cells as shown by 3-­‐(4,5-­‐ dimethylthiazol-­‐2-­‐yl)-­‐2,5-­‐diphenyltetrazolium bromide assay. The differentiation capacity of hASCs seeded on scaffolds was confirmed by the reduction of the proliferation, the alkaline phosphatase (AP) activity, expression of osteogenic gene markers (AP, collagen type I, Runx2, and osteocalcin), and the expression of bone markers, such as osteopontin, osteocalcin, and collagen type I. The osteogenic capacity of hASCs seeded on PHB-­‐HV scaffolds indicates that this scaffold is adequate for cell growth and differentiation and that aHS is
TypeArticle
URIhttp://hdl.handle.net/1822/23906
DOI10.1089/ten.tea.2012.0189
ISSN1937-3341
Publisher versionhttp://online.liebertpub.com/doi/abs/10.1089/ten.tea.2012.0189
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
17462-Human adipose.pdf
  Restricted access
23,79 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID