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TitleLoss of RKIP expression during the carcinogenic evolution of endometrial cancer
Author(s)Martinho, Olga
Faloppa, Carlos
Neto, Cristovam Scapulatempo
Longatto Filho, Adhemar
Baiocchi, Glauco
Cunha, Isabela Werneck da
Soares, Fernando Augusto
Fregnani, José Humberto
Reis, R. M.
Issue dateFeb-2012
PublisherBMJ Publishing Group
JournalJournal of Clinical Pathology
Abstract(s)Aims: Endometrial cancer is one of the most common cancers in women worldwide, but there is a lack of diagnostic markers for early detection of these tumours. The raf kinase inhibitory protein (RKIP) negatively regulates the Raf/MEK/ERK pathway, and the downregulation of RKIP is associated with tumour progression and metastasis in several human neoplasms. The aim of this study was to assess the expression levels of RKIP in endometrial cancer and determine whether this expression correlates with clinical outcome in these patients. Methods: Tissue microarrays constructed using tissue samples from 209 endometrial adenocarcinomas, 49 endometrial polyps and 48 endometrial hyperplasias were analysed for RKIP expression by immunohistochemistry. Results: The authors found that RKIP expression decreases significantly during malignant progression of endometrial cancer; it is highly expressed in non-neoplastic tissues (polyps 79.6%; hyperplasias 87.5%) and expressed at very low levels in endometrioid adenocarcinomas (29.7%). No correlations were observed between RKIP expression, clinicopathological data and survival. Conclusion: This study demonstrated for the first time that RKIP expression is lost during the carcinogenic evolution of endometrial tumours and that the loss of RKIP expression is associated with a malignant phenotype. Functional studies are needed to address the biological role of RKIP downregulation in endometrial cancer.
DescriptionOM performed the immunohistochemical reactions and drafted the manuscript. CCF, IWC and FAS constructed the tissue microarray. CSN and ALF evaluated the immunohistochemistry reactions. CCF, GB, IWC, FAS and JHTGF were involved in collection of clinicalepathological data and collaborated on the data interpretation. JHTGF and RMR were involved in the study concept and design, study supervision, statistical analysis and critical revision of the manuscript. All the authors have read and approved the final manuscript.
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Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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