Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/23345

TitlePhotocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
Author(s)Mihaila, Silvia M.
Gaharwar, Akhilesh K.
Reis, R. L.
Marques, A. P.
Gomes, Manuela E.
Khademhosseini, Ali
KeywordsHydrogels
Patterning
Photocrosslinkable Kappa-Carrageenan
Tissue engineering
kappa-carrageenan
mechanical properties
polymeric materials
Issue date2013
PublisherWiley
JournalAdvanced Healthcare Materials
Abstract(s)Kappa carrageenan ( κ -CA) is a natural-origin polymer that closely mimics the glycosaminoglycan structure, one of the most important constituents of native tissues extracellular matrix. Previously, it has been shown that κ -CA can crosslink via ionic interactions rendering strong, but brittle hydrogels. In this study, we introduce photocrosslinkable methacrylate moieties on the κ -CA backbone to create physically and chemically crosslinked hydrogels highlighting their use in the context of tissue engineering. By varying the degree of methacrylation, the effect on hydrogel crosslinking was investigated in terms of hydration degree, dissolution profi les, morphological, mechanical, and rheological properties. Furthermore, the viability of fi broblast cells cultured inside the photocrosslinked hydrogels was investigated. The combination of chemical and physical crosslinking procedures enables the formation of hydrogels with highly versatile physical and chemical properties, while maintaining the viability of encapsulated cells. To our best knowledge, this is the fi rst study reporting the synthesis of photocrosslinkable κ -CA with controllable compressive moduli, swelling ratios and pore size distributions. Moreover, by micromolding approaches, spatially controlled geometries and cell distribution patterns could be obtained, thus enabling the development of cell-material platforms that can be applied and tailored to a broad range of tissue engineering strategies.
TypeArticle
DescriptionPublished online
URIhttp://hdl.handle.net/1822/23345
DOI10.1002/adhm.201200317
ISSN2192-2640
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/adhm.201200317/abstract
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals


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