Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/23327

TitleSurface microstructuring and protein patterning using hyaluronan derivatives
Author(s)Márquez-Posadas, M. C.
Ramiro, J.
Becher, J.
Yang, Y.
Köwitsch, A.
Pashkuleva, I.
Díez-Ahedo, R.
Schnabelrauch, Matthias
Reis, R. L.
Groth, T.
Merino, S.
KeywordsGlycosaminoglycan
Hyaluronan
Hydrogel
Microcontact printing
Microstructuring
Photocrosslinking
Issue dateFeb-2013
PublisherElsevier
JournalMicroelectronic Engineering
Abstract(s)Natural polymers, such as hyaluronan, are considered as good candidates to substitute synthetic materials in many biological applications due to their intrinsic biocompatibility for in vitro and living cell experiments. This work describes surface modifications performed over modified hyaluronan derivatives which could help to understand the physical and biochemical cues on the cell-behaviour. A photocrosslinkable methacrylated hyaluronan was microstructured using soft lithography techniques to obtain a microenvironment suitable for cell-behaviour experiments, which might mimic the extra-cellular matrix. In addition, sulphated and non-sulphated oxidized hyaluronan were immobilized on bare substrates and micrometric features of cell adhesive and non-adhesive proteins were patterned by microcontact printing on top of them. The obtained structures were characterized by optical microscopy, profilometry and atomic force microscopy. The stability of structures was tested by immersion in physiological salt solutions. The observed results prove the suitability of the materials and protocols described.
Typearticle
URIhttp://hdl.handle.net/1822/23327
DOI10.1016/j.mee.2013.01.039
ISSN0167-9317
Publisher versionhttp://www.sciencedirect.com/science/article/pii/S0167931713000671
Peer-Reviewedyes
AccessrestrictedAccess
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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