Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/23153

TítuloEvaluation of different formulations of gellan gum-based hydrogels for tissue engineering of intervertebral disc
Autor(es)Lee, S. K.
Correia, Joana Silva
Caridade, S. G.
Mano, J. F.
Oliveira, Joaquim M.
Khang, G.
Reis, R. L.
Palavras-chaveGellan Gum
Intervertebral disc
DataSet-2012
EditoraJohn Wiley & Sons
Resumo(s)Gellan gum based-hydrogels present advantageous features for application as acellular and cellular nucleus pulposus (NP) substitutes due to the possibility of fine-tuning its physico-chemical and biological properties. In this study, ionic-crosslinked hydrogel discs were produced by means of mixing a raw and chemically modified material, i.e., high acyl gellan gum (HAGG) and methacrylated low acyl gellan gum (GG-MA), respectively. The hydrogel discs were characterized in terms of its mechanical properties and degradation/swelling ability. The biocompatibility of the different hydrogel formulations was assessed in vitro using NP rabbit cells isolated from the intervertebral disc. The biological performance of the developed gellan gum-based hydrogels formulations was evaluated by: (i) culturing of NP cells in the presence of the hydrogel leachables, and (ii) seeding or encapsulation of the NP cells within the hydrogels. The present work demonstrated that as HAGG content increases, the modulus of the hydrogels decreases. Moreover, the increase of the HAGG content induces a higher weight loss of the GG-MA/HA-GG formulation as compared to GG-MA hydrogel. The in vitro study revealed that hydrogels are non-cytotoxic and support the encapsulation of rabbit NP cells. The methacrylated gellan gum and formulations possessing high acyl gellan gum present tunable properties that may be interesting for application as NP substitutes.
TipoconferenceAbstract
URIhttp://hdl.handle.net/1822/23153
ISSN1932-7005
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
1347274077_term1586_44.pdf60,2 kBAdobe PDFVer/Abrir

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis