Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/22516

TitleBiohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures
Author(s)Abreu, A. A.
Karakashev, Dimitar
Angelidaki, I.
Sousa, D. Z.
Alves, M. M.
KeywordsExtreme thermophilic conditions
Biohydrogen
Arabinose
Hydrogen partial pressure
pH
Lactate
Issue date2012
PublisherBioMed Central (BMC)
JournalBiotechnology for Biofuels
Abstract(s)Background: Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of lingocellulosic materials is essential for the success of these processes. Results: Conversion of arabinose and glucose to hydrogen, by extreme thermophilic anaerobic mixed cultures was studied in continuous (70ºC, pH 5.5) and batch (70ºC, pH 5.5 and pH 7) assays. Two EGSB reactors, Rarab and Rgluc, were continuously fed with arabinose and glucose, respectively. No significant differences in reactor performance were observed for arabinose and glucose organic loading rates (OLR) ranging from 4.3 to 7.1 kgCOD m-3 d-1. However, for an OLR of 14.2 kgCOD m-3 d-1, hydrogen production rate and hydrogen yield were higher in Rarab than in Rgluc (average hydrogen production rate of 3.2 and 2.0 LH2 L-1 d-1 and hydrogen yield of 1.10 and 0.75 molH2 mol-1substrate for Rarab and Rgluc, respectively). Lower hydrogen production in Rgluc was associated with higher lactate production. DGGE results revealed no significant difference on the bacterial community composition between operational periods and between the reactors. Increased hydrogen production was observed in batch experiments when hydrogen partial pressure was kept low, both with arabinose and glucose as substrate. Sugars were completely consumed and hydrogen production stimulated (62% higher) when pH 7 was used instead of pH 5.5. Conclusions: Continuous hydrogen production rate from arabinose was significantly higher than from glucose, when higher organic loading rate was used. The effect of hydrogen partial pressure on hydrogen production from glucose in batch mode was related to the extent of sugar utilization and not to the efficiency of substrate conversion to hydrogen. Furthermore, at pH 7.0, sugars uptake, hydrogen production and yield were higher than at pH 5.5, with both arabinose and glucose as substrates.
TypeArticle
URIhttp://hdl.handle.net/1822/22516
DOI10.1186/1754-6834-5-6
ISSN1754-6834
Publisher versionhttp://www.biotechnologyforbiofuels.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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