Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/22504

TitleEffect of pulsed electric field on the germination of barley seeds
Author(s)Dymek, K.
Dejmek, Petr
Panarese, V.
Vicente, A. A.
Wadsö, Lars
Finnie, C.
Gómez Galindo, Federico
KeywordsIsothermal calorimetry
Radical emergence
alpha-Amylase
Issue date2012
PublisherElsevier
JournalLWT - Food Science and Technology
Abstract(s)This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEF). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEF affects radicle emergence without significantly affecting the seeds’ gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection limit. However, western blotting demonstrated that α-amylase concentration decreases in the PEF-treated seeds.
TypeArticle
URIhttp://hdl.handle.net/1822/22504
DOI10.1016/j.lwt.2011.12.019
ISSN0023-6438
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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