Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/22440

TitleFunctionalization of wool with L-cysteine : process characterization and assessment of antimicrobial activity and cytotoxicity
Author(s)Gouveia, Isabel
Sá, Daniela
Henriques, Mariana
KeywordsAntimicrobial textiles
Biopolymers
S. aureus
antimicrobial textiles; biopolymers; S. aureus;
K. pneumoniae
Sulfhydryl groups
Ellman’s reagent
S
aureus
K
pneumoniae
Issue date2012
PublisherJohn Wiley and Sons
JournalJournal of Applied Polymer Science
Abstract(s)This investigation reports a new biotechnological process that uses L-cysteine (L-Cys) which provides a permanent, nontoxic and effective antimicrobial effect over wool-based materials. This process is simple and carried out via idespread exhausting methods. Typically, wool fabrics are incubated with L-Cys for 50 min at 60°C in a pH 4.8 acetate buffer solution 25 mM, under mild agitation to give a good absorption rate. The minimal inhibitory concentration (MIC) of L-Cys was evaluated by the NCCLS M07-A6 standard method, and the results showed a good antibacterial activity against S. aureus and K. pneumoniae within the range of 6.0 × 10−3 − 4.8 × 10−2 g/mL [MIC 0.6% (w/v)] and 6.0 × 10−3 − 4.8 × 10−2 g/mL [MIC 0.6% (w/v)], respectively. In addition, the antimicrobial activity of the functionalized wool was assessed by the international standard JIS 1902-2002 showing a good inhibition of bacterial growth for an L-Cys concentration of 1% over the weight of fabric, both against Staphylococcus aureus and Klebsiella pneumoniae. Moreover, the biocidal mechanism was found to be related with the increase of sulfhydryl's groups onto wool fibers, which were quantified by the Ellman's reagent (5,5′-Dithio-bis(2-nitrobenzoic acid) method. The new process is easy to perform, non toxic, preserve wool quality and is a novel biomimetic approach that uses antimicrobial amino acids and may open new avenues for the design of biomedical textiles with a broad range of applications in healthcare.
TypeArticle
URIhttp://hdl.handle.net/1822/22440
DOI10.1002/app.34587
ISSN0021-8995
Other identifiers1097-4628
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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