Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/22394

TitleCandida tropicalis biofilms : effect on urinary epithelial cells
Author(s)Negri, M.
Silva, Sónia Carina
Brêda, D.
Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
KeywordsCandida tropicalis
Biofilm
Artificial urine
Adhesion
Epithelial cells
SAP gene
Issue date2012
PublisherElsevier
JournalMicrobial Pathogenesis
Abstract(s)Candida tropicalis infection is strongly associated with the presence of biofilms in urinary catheters. Thus, the aim of this work was to study the behaviour of C. tropicalis in biofilms of different ages (24e120 h) formed in artificial urine (AU) and their effect in human urinary bladder cells (TCC-SUP). Reference strain ATCC 750 and two isolates from patients with candiduria (U69 and U75) were used in this study. The adhesion to human cells was evaluated after 2 h of contact with Candida biofilms, using the Crystal violet staining method, and the human cells response was evaluated in terms of activity inhibition and cell damage. Candida tropicalis aspartyl proteinase (SAPT) gene expression was determined by real-time PCR. Candida tropicalis biofilm cells were able to adhere to TCC-SUP cells. The highest extent of yeast attachment was obtained for the 72 h old biofilm cells. Yeasts affected TCC-SUP cells, with 120 h-biofilm cells causing the highest levels of cell injury. Generally, SAPT3 was highly expressed and SAPT4 was only detected in the reference strain. Overall, it is important to highlight that C. tropicalis cells detached from biofilms are able to colonize human cells and cause some injury and reduction of metabolic activity.
TypeArticle
URIhttp://hdl.handle.net/1822/22394
DOI10.1016/j.micpath.2012.05.006
ISSN0882-4010
1096-1208
Publisher versionhttp://www.sciencedirect.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
1-s2.0-S088240101200109X-main.pdf178,18 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID