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|Title:||Applying a metabolic footprinting approach to characterize the impact of the recombinant protein production in Escherichia coli|
Villas-Bôas, S. G.
Ferreira, E. C.
|Journal:||Advances in Intelligent and Soft Computing|
|Abstract(s):||In this study metabolic footprinting was applied to evaluate the metabolic consequences of protein overproduction at slow growth conditions (μ = 0.1 h-1). The extracellular metabolites detected by gas chromatography-mass spectrometry characterized the metabolic footprints before and after the induction of the recombinant protein production (i.e. pre- and post-induction phases). Metabolic footprinting enabled the discrimination between the two growth phases and ex-posed significant metabolic alterations in the extracellular milieu during the re-combinant processes.|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|
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