Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/22076

TitleAn ubiquitous perspective of the intraspecific diversity of the aquatic fungus articulospora tetracladia
Author(s)Seena, Sahadevan
Duarte, Sofia Alexandra Ferreira
Pascoal, Cláudia
Cássio, Fernanda
Issue dateJul-2012
Abstract(s)The cosmopolitan Articulospora tetracladia is a dominant sporulating species on decomposing plant-litter in streams of Northwest Iberian Peninsula. In an attempt to elucidate the intraspecific genetic diversity of A. tetracladia of Iberian Peninsula, isolates were attained from various types of substrates from streams of North and Central Portugal and North Spain, between 2000 and 2010. Genetic diversity of these fungal populations was assessed by denaturing gradient gel electrophoresis (DGGE) fingerprints and by using ITS1-5.8S-ITS2 barcodes. The ITS1-5.8S-ITS2 barcodes published at the National Center for Biotechnology (NCBI) or National Institute of Technology and Evaluation Biological Resource Center (NBRC) were retrieved to probe into the genetic diversity of A. tetracladia isolated from Iberian Peninsula and other parts of the world (Central Europe, UK, Canada, Japan and Malaysia). The PCR-DGGE of ITS2 region of 50 Iberian fungal isolates distinguished 8 OTUs. The ITS sequences of 68 fungal isolates yielded nine OTUs, but 5 fungal isolates were not assigned to any of these OTUs. The A. tetracladia strains did not exhibit cohesiveness based on sampling date or substrate or geographic location. Overall results indicate that, apart Malaysian genotypes, A. tetracladia genotypes are geographically widespread irrespective of sampling time, sites or substrates. Moreover, PCR-DGGE appeared to be a rapid tool for assessing intraspecific diversity of aquatic hyphomycetes.
TypeAbstract
URIhttp://hdl.handle.net/1822/22076
Peer-Reviewedno
AccessOpen access
Appears in Collections:DBio - Comunicações/Communications in Congresses

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