Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/22006

TitleAminodi(hetero)arylamines in the thieno[3,2-b]pyridine series: synthesis, effects in human tumor cells growth, cell cycle analysis, apoptosis and evaluation of toxicity using non-tumor cells
Author(s)Calhelha, Ricardo C.
Ferreira, Isabel C. F. R.
Peixoto, Daniela
Abreu, Rui M. V.
Silva, L. A. Vale
Pinto, Eugénia
Lima, Raquel T.
Alvelos, Maria I.
Vasconcelos, M. Helena
Queiroz, Maria João R. P.
KeywordsThieno[3,2-b]pyridines
Aminodi(hetero)arylamines
Buchwald-hartwig C-N coupling
Antitumoral activity
Toxicity
Cell cycle
Apoptosis
2-b]pyridines
Thieno[3
Issue date28-Mar-2012
PublisherMDPI
JournalMolecules
Abstract(s)Three aminodi(hetero)arylamines were prepared via a palladium-catalyzed C-N Buchwald-Hartwig coupling of methyl 3-aminothieno[3,2-b]pyridine-2-carboxylate with different bromonitrobenzenes, followed by reduction of the nitro groups of the coupling products to the corresponding amino compounds. The aminodi(hetero)arylamines thus obtained were evaluated for their growth inhibitory effect on four human tumor cell lines MCF-7 (breast adenocarcinoma), A375-C5 (melanoma), NCI-H460 (non-small cell lung cancer) and HepG2 (hepatocellular carcinoma). The toxicity to non-tumor cells was also evaluated using a porcine liver primary cell culture (PLP1), established by us. The aminodi(hetero)arylamine with the NH2 group in the ortho position and an OMe group in the para position to the NH of the di(hetero)arylamine, is the most promising compound giving the lowest GI50 values (1.30–1.63 μM) in all the tested human tumor cell lines, presenting no toxicity to PLP1 at those concentrations. The effect of this compound on the cell cycle and induction of apoptosis was analyzed in the NCI-H460 cell line. It was observed that it altered the cell cycle profile causing a decrease in the percentage of cells in the G0/G1 phase and an increase of the apoptosis levels.
TypeArticle
URIhttp://hdl.handle.net/1822/22006
DOI10.3390/molecules17043834
ISSN1420-3049
Publisher versionwww.mdpi.com/journal/molecules
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CDQuim - Artigos (Papers)

Files in This Item:
File Description SizeFormat 
molecules-17-03834[1].pdfArtigo publicado em open-acess186,95 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID