Please use this identifier to cite or link to this item:

TitleAbsorption and fluorescence studies of new 3-amino-N-(2-hydroxy-, (3-hydroxypropoxy)- or (3-chloropropoxy)-5-(propylamino)-9H-benzo[a]phenoxazin-9-ylidene)propan-1-aminium chlorides
Author(s)Firmino, A. D. G.
Gonçalves, M. Sameiro T.
Fluorescent probes
Long-wavelength fluorophores
Issue dateJul-2012
Abstract(s)In recent years, fluorescence techniques have seen an enormous improvement, being in nowadays an interesting tool in the visualization and quantification of biomolecules, frequently used in cell biology, biochemistry, proteomics, immunochemistry, flow cytometry and medical diagnostics.1 Although intrinsic fluorophores are of great interest, the development of new extrinsic fluorophores is crucial for the design of new fluorescent probes, namely with improved water solubility, functional groups, and enhanced fluorescence quantum efficiency at high emission wavelengths.2 As a continuation of our previous research,3 this communication describes the evaluation of absorption and emission properties of new mono- and bifunctionalised benzo[a]phenoxazinium chlorides 1a-c in ethanol, water, and at simulated physiological pH, focus on the variation of the concentration. In order to investigate the sensitivity of fluorescence of the long-wavelength fluorophores to pH, photophysical studies under controlled pH also were performed.
AccessRestricted access (UMinho)
Appears in Collections:CDQuim - Comunicações e Proceedings

Files in This Item:
File Description SizeFormat 
Abstract - II_ETCAQ- ADF.pdf
  Restricted access
Final draft post-refereeing65,22 kBAdobe PDFView/Open    Request a copy!

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID