Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/21137

TitleBiocompatibility of mannan nanogel : safe interaction with plasma proteins
Author(s)Ferreira, Sílvia A.
Oslakovic, Cecilia
Cukalevski, Risto
Frohm, Birgitta
Dahlbäck, Björn
Linse, Sara
Gama, F. M.
Cedervall, Tommy
KeywordsMannan nanogel
Protein corona
Coagulation
Fibrillation
Biosafety
Biocompatibility
Issue date2012
PublisherElsevier
JournalBiochimica et Biophysica Acta (BBA). General Subjects
Abstract(s)BACKGROUND: Self-assembled mannan nanogels are designed to provide a therapeutic or vaccine delivery platform based on the bioactive properties of mannan to target mannose receptor expressed on the surface of antigen-presenting cells, combined with the performance of nanogels as carriers of biologically active agents. METHODS: Proteins in the corona around mannan nanogel formed in human plasma were identified by mass spectrometry after size exclusion chromatography or centrifugation followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Structural changes and time dependent binding of human apolipoprotein A-I (apoA-I) and human serum albumin (HSA) to mannan nanogel were studied using intrinsic tryptophan fluorescence and circular dichroism spectroscopy. The mannan nanogel effect on blood coagulation and fibrillation of Alzheimer's disease-associated amyloid β peptide and hemodialysis-associated amyloidosis β2 microglobulin was evaluated using thrombin generation assay or thioflavin T fluorescence assay, respectively. RESULTS: The protein corona around mannan nanogel is formed through a slow process, is quite specific comprising apolipoproteins B-100, A-I and E and HSA, evolves over time, and the equilibrium is reached after hours to days. Structural changes and time dependent binding of apoA-I and HSA to mannan nanogel are minor. The mannan nanogel does not affect blood coagulation and retards the fibril formation. CONCLUSIONS: Mannan nanogel has a high biosafety and biocompatibility, which is mandatory for nanomaterials to be used in biomedical applications. GENERAL SIGNIFICANCE: Our research provides a molecular approach to evaluate the safety aspects of nanomaterials, which is of general concern in society and science.
TypeArticle
URIhttp://hdl.handle.net/1822/21137
DOI10.1016/j.bbagen.2012.04.015
ISSN0304-4165
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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