Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20652

TitleDynamic culture of osteogenic cells in biomimetically coated poly(caprolactone) nanofibre mesh constructs
Author(s)Araújo, J. V.
Reis, C. Cunha
Rada, Tommaso
Silva, Marta Alves da
Gomes, Manuela E.
Yang, Y.
Ashammakhi, N.
Reis, R. L.
El-Haj, Alicia J.
Neves, N. M.
Issue date2010
PublisherMary Ann Liebert
JournalTissue engineering. Part A
Abstract(s)In our previous work, biomimetic calcium phosphate-coated poly(caprolactone) nanofibre meshes (BCP-NMs) were demonstrated to be more effective for supporting cell attachment and proliferation under static conditions, when compared with poly(caprolactone) nanofibre meshes (PCL-NMs). In many applications, in vitro cultivation of constructs using bioreactors that support efficient nutrition of cells has appeared as an important step toward the development of functional grafts. This work aimed at studying the effects of dynamic culture conditions and biomimetic coating on bone cells grown on the nanofibre meshes. BCP-NM and PCL-NM were seeded with osteoblast-like cells (MG63--human osteosarcoma-derived cell line). The cell-seeded constructs were cultured within a rotating bioreactor that simulated microgravity, at a fixed rotating speed, for different time periods, and then characterized. Cell morphology, viability, and phenotype were assessed. PCL-NM constructs presented a higher number of dead cells than BCP-NM constructs. Under dynamic conditions, the production of proteins associated with the extracellular matrix of bone was higher on BCP-NM constructs than in the PCL-NM ones, which indicates that coated samples may provide cells with a better environment for tissue growth. It is suggested that improved mass transfer in the bioreactor in combination with the appropriate substrate were decisive factors for this highly positive outcome for generating bone.
TypeArticle
URIhttp://hdl.handle.net/1822/20652
DOI10.1089/ten.tea.2009.0223
ISSN1937-3341
1937-335X
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
file.pdf450,54 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID