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TitleSilk fibroin microparticles as carriers for delivery of human recombinant bone morphogenetic protein-2 : in vitro and in vivo bioactivity
Author(s)Bessa, Paulo C.
Balmayor, Elizabeth Rosado
Hartinger, Joachim
Zanoni, Gerald
Dopler, Daniela
Meinl, Alexandra
Banerjee, Asmita
Casal, Margarida
Redl, Heinz
Reis, R. L.
Griensven, Martijn van
Issue date2010
PublisherMary Ann Liebert
JournalTissue Engineering : Part C, Methods
Abstract(s)The in vitro and in vivo efficiency of fibroin microparticles as a delivery carrier for bone morphogenetic protein-2 (BMP-2) was evaluated. BMP-2 was encapsulated in silk fibroin particles that were produced by a simple and very mild processing method. The dose-response of BMP-2-loaded fibroin particles was examined in C2C12 cells, after 5 days of culture. The BMP-2 retained most of its activity as observed by the increase in alkaline phosphatase activity, which was much higher when BMP-2 was encapsulated into the particles rather than just surface-adsorbed. After 2 weeks of culture, increased mineralization was observed with BMP-2-loaded particles in comparison to soluble added growth factor. No significant cytotoxicity was detected. When implanted in a rat ectopic model, bone formation was observed by in vivo micro-computed tomography after 2 and 4 weeks postimplantation, with particles loaded with 5 or 12.5 microg BMP-2. An increase in bone density was observed over time. Histology revealed further evidence of ectopic bone formation, observed by strong alizarin red staining and osteocalcin immunostaining. Our findings show that fibroin microparticles may present an interesting option for future clinical applications in the bone tissue engineering field, and therefore, further studies have been planned.
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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