Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20590

TitleInjectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects
Author(s)Oliveira, João T.
Gardel, Leandro. S.
Rada, Tommaso
Martins, L.
Gomes, Manuela E.
Reis, R. L.
KeywordsGellan gum
Stem cells
Cartilage
Tissue engineering
In vivo
Issue dateSep-2010
PublisherWiley
JournalJournal of Orthopaedic Research
Abstract(s)In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellangumwith encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASCþGF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsulated rabbit articular chondrocytes (AC) (standard control); (d) gellan gum alone (control); (e) empty defect (control). Fullthickness articular cartilage defects were created and the gellangum constructs were injected and left for 8 weeks. The macroscopic aspect of the explants showed a progressive increase of similarity with the lateral native cartilage, stable integration at the defect site, more pronouncedly in the cell-loaded constructs. Tissue scoring showed that ASCþGF exhibited the best results regarding tissue quality progression. Alcian blue retrieved similar results with a better outcome for the cell-loaded constructs. Regarding real-time PCR analyses, ASCþGF had the best progression with an upregulation of collagen type II and aggrecan, and a downregulation of collagen type I. Gellan gum hydrogels combined with autologous cells constitute a promising approach for the treatment of articular cartilage defects, and adipose derived cellsmayconstitute a valid alternative to currently used articular chondrocytes.
TypeArticle
URIhttp://hdl.handle.net/1822/20590
DOI10.1002/jor.21114
ISSN0736-0266
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/jor.21114/pdf
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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