Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20269

TitleStraightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
Author(s)Silva, Ricardo M. P. da
Mano, J. F.
Reis, R. L.
Keywordsacetylation
chitin
chitosan
deacetylation
NMR
UV spectrophotometry
Issue date2008
PublisherWiley
JournalMacromolecular Chemistry and Physics
Abstract(s)First-derivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of N-acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the first derivative of its UV spectrum at 202 nm, thus eliminating the need for empiric correction curves for highly deacetylated samples. A procedure is proposed for the accurate mass determination of the hygroscopic chitosan. The proposed approach facilitates the routine determination of the DA, especially when using potent multiwell microplate readers, which allow hundreds of samples to be measured in just a few minutes.
TypeArticle
URIhttp://hdl.handle.net/1822/20269
DOI10.1002/macp.200800191
ISSN1521-3931
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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