Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20254

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dc.contributor.authorPinto, A. R.-
dc.contributor.authorSalgado, A. J.-
dc.contributor.authorCorrelo, V. M.-
dc.contributor.authorSol, P.-
dc.contributor.authorBhattacharya, Mrinal-
dc.contributor.authorCharbord, Pierre-
dc.contributor.authorReis, R. L.-
dc.contributor.authorNeves, M. N.-
dc.date.accessioned2012-09-18T11:02:42Z-
dc.date.available2012-09-18T11:02:42Z-
dc.date.issued2008-
dc.identifier.issn2152-4947por
dc.identifier.urihttp://hdl.handle.net/1822/20254-
dc.description.abstractThe aim of the present work was to study the biological behavior of a mouse mesenchymal stem cell line when seeded and cultured under osteogenic conditions onto novel processed melt-based chitosan scaffolds. Scaffolds were produced by compression molding, followed by salt leaching. Scanning electron microscopy (SEM) observations and lCT analysis showed the pore sizes ranging between 250 and 500 lm and the interconnectivity of the porous structure. The chitosan–poly(butylenes succinate) scaffolds presented high mechanical properties, similar to the ones of trabecular bone (E1%*75 MPa). Cytotoxicity assays were carried out using standard tests (accordingly to ISO/EN 10993 part 5 guidelines), namely, MTS test with a 24 h extraction period, revealing that L929 cells had similar metabolic activities to that obtained for the negative control. Cell culture studies were conducted using a mouse mesenchymal stem cell line (BMC9). Cells were seeded onto the scaffold and allowed to proliferate for 3 weeks under osteogenic conditions. SEM observations demonstrated that cells were able to proliferate and massively colonize the scaffolds structure. The cell viability assay MTS demonstrated that BMC9 cells were viable after 3 weeks of culture. The cells clearly evidenced a positive differentiation toward the osteogenic lineage, as confirmed by the high ALP activity levels. Moreover, energy dispersive spectroscopy (EDS) analysis revealed the presence of Ca and P in the elaborated extracellular matrix (ECM). These combined results indicate that the novel melt-based chitosan/polyester scaffolds support the adhesion, proliferation, and osteogenic differentiation of the mouse MSCs and shows adequate physicochemical and biological properties for being used as scaffolds in bone tissue engineering–related strategies.por
dc.language.isoengpor
dc.publisherMary Ann Liebertpor
dc.rightsopenAccesspor
dc.titleAdhesion, proliferation, and osteogenic differentiation of a mouse mesenchymal stem cell line (BMC9) seeded on novel melt-based chitosan/polyester 3D porous scaffoldspor
dc.typearticlepor
dc.peerreviewedyespor
sdum.publicationstatuspublishedpor
oaire.citationStartPage1049por
oaire.citationEndPage1057por
oaire.citationIssue6por
oaire.citationTitleTissue Engineering : part Apor
oaire.citationVolume14por
dc.identifier.doi10.1089/ten.tea.2007.0153por
dc.subject.wosScience & Technologypor
sdum.journalTissue Engineering : part Apor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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