Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20193

TitleGAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment
Author(s)Francesko, Antonio
Costa, Diana Soares da
Lisboa, P.
Reis, R. L.
Pashkuleva, I.
Tzanov, Tzanko
Issue dateJun-2012
PublisherRoyal Society of Chemistry
JournalJournal of Materials Chemistry
Abstract(s)Multilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surfaces was monitored in situ by QCM-D. All experimental groups showed exponential growth of the coatings controlled by the degree of chitosan thiolation and the molecular weight of the GAGs. The degree of chitosan modification was also the key parameter influencing the enzyme activity: increasing the thiols content led to more efficient myeloperoxidase inhibition and was inversely proportional to the adsorption of collagenase. Enhanced fibroblast attachment and proliferation were observed when the multilayered polyelectrolyte constructs terminated with GAGs. The possibility to control either the activity of major wound enzymes by the thiolation degree of the coating or the cell adhesion and proliferation by proper selection of the ultimate layer makes these materials potentially useful in chronic wounds treatment and dermal tissue regeneration.
TypeArticle
URIhttp://hdl.handle.net/1822/20193
DOI10.1039/c2jm31051a
ISSN0959-9428
Publisher versionhttp://pubs.rsc.org/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals
ICVS - Artigos em revistas internacionais / Papers in international journals

Files in This Item:
File Description SizeFormat 
Francesco et al_JMatChem_2012.pdfDocumento principal456,39 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID