Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/19951

TitleWave characterization for mammalian cell culture : residence time distribution
Author(s)Rodrigues, E.
Costa, A. R.
Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
KeywordsWave bioreactor
Stirred tank bioreactor
Residence time distribution
Ideal RTD models
Mammalian cell culture
Issue date2012
PublisherElsevier
JournalNew Biotechnology
Abstract(s)The high dose requirements of biopharmaceutical products led to the development of mammalian cell culture technologies that increase biomanufacturing capacity. The disposable Wave bioreactor is one of the most promising technologies, providing ease of operation and no cross-contamination, and using an innovative undulation movement that ensures good mixing and oxygen transfer without cell damage. However, its recentness demands further characterization. This study evaluated the residence time distribution (RTD) in Wave, allowing the characterization of mixing and flow and the comparison with ideal models and a Stirred tank reactor (STR) used for mammalian cell culture. RTD was determined using methylene blue with pulse input methodology, at three flow rates common in mammalian cell culture (3.3 × 10−5 m3/h, 7.9 × 10−5 m3/h, and 1.25 × 10−4 m3/h) and one typical of microbial culture (5 × 10−3 m3/h). Samples were taken periodically and the absorbance read at 660 nm. It was observed that Wave behavior diverted from ideal models, but was similar to STR. Therefore, the deviations are not related to the particular Wave rocking mechanism, but could be associated with the inadequacy of these reactors to operate in continuous mode or to a possible inability of the theoretical models to properly describe the behavior of reactors designed for mammalian cell culture. Thus, the development of new theoretical models could better characterize the performance of these reactors.
Typearticle
URIhttp://hdl.handle.net/1822/19951
DOI10.1016/j.nbt.2011.10.006
ISSN1871-6784
Publisher versionhttp://www.sciencedirect.com/
Peer-Reviewedyes
AccessrestrictedAccess
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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