Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/19935

TitleNew improved method for fructooligosaccharides production by Aureobasidium pullulans
Author(s)Dominguez, Ana
Nobre, C.
Rodrigues, L. R.
Peres, A. M.
Torres, D.
Rocha, I.
Lima, Nelson
Teixeira, J. A.
KeywordsFructooligosaccharides
Aureobasidium pullulans
Transfructosylation
One-stage fermentation process
Response surface methodology
Product yield
Issue date2012
PublisherElsevier
JournalCarbohydrate Polymers
Abstract(s)Fructooligosaccharides are prebiotics with numerous health benefits within which the improvement of gut microbiota balance can be highlighted, playing a key role in individual health. In this study, an integrated one-stage method for FOS production via sucrose fermentation by Aureobasidium pullulans was developed and optimized using experimental design tools. Optimization of temperature and agitation speed for maximizing the FOS production was performed using response surface methodology. Temperature was found to be the most significant parameter. The optimum fermentation conditions were found to be 32 ◦C and 385 rpm. Under these conditions, the model predicted a total FOS production yield of 64.7 gFOS/gsucrose. The model was validated at optimal conditions in order to check its adequacy and accuracy and an experimental yield of 64.1 (±0.0) gFOS/gsucrose was obtained. A significant improvement of the total FOS production yields by A. pullulans using a one-stage process was obtained
TypeArticle
URIhttp://hdl.handle.net/1822/19935
DOI10.1016/j.carbpol.2012.03.091
ISSN0144-8617
Publisher versionwww.elsevier.com/locate/carbpol
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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