Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/19930

TitlePerformance of a biosurfactant produced by a Bacillus subtilis strain isolated from crude oil samples as compared to commercial chemical surfactants
Author(s)Altmajer Vaz, Deisi
Gudiña, Eduardo J.
Jurado Alameda, Encarnacion
Teixeira, J. A.
Rodrigues, L. R.
KeywordsBiosurfactants
Bacillus subtilis
Glucopone®215
Glucopone®650
Findet®1214N/23
LAS
Glucopone (R) 215
Glucopone (R) 650
Findet (R) 1214N/23
Issue date2012
PublisherElsevier
JournalColloids and Surfaces B : Biointerfaces
Abstract(s)In this work, the biosurfactant produced by a Bacillus subtilis strain isolated from crude oil samples was characterized and its properties compared with commercially available chemical surfactants. The purified biosurfactant production yield (YP/X) was 0.20 g/g cell dry weight. The surface tension (29.0 mN/m) and critical micelle concentration (40 mg/l) were found to be similar to the values previously reported for surfactin. Temperature and pH stability of the biosurfactant were also evaluated. The biosurfactant was exposed to different temperatures (20, 37 and 46 ◦C) during two weeks and was found to be as stable as the commercial chemical surfactants Glucopone®215, Glucopone®650, Findet®1214N/23 and linear alkylbenzene sulfonates (LAS). Moreover, the biosurfactant subjected to 121 ◦C for 20 min did not exhibit a significant loss of surface activity. Contrary to the commercial chemical surfactants that were found to be stable over a wide range of pH (3.0–10.0), the biosurfactant was unstable precipitating at pH values below 5.0. The emulsification indexes showed that the biosurfactant possesses equal or superior capacity to form emulsions with n-hexadecane as compared to the commercial chemical surfactants. Moreover, the anti-adhesive activity of the biosurfactant and commercial chemical surfactants was evaluated. The biosurfactant showed some activity against Staphylococcus aureus and Escherichia coli. Nevertheless, no particular trend or special effect could be assigned to the use of commercial chemical surfactants as anti-adhesives. Results gathered in this work suggest that the biosurfactant recovered from B. subtilis EG1 constitutes an interesting alternative to the commercial chemical surfactants with potential use in several industries
TypeArticle
URIhttp://hdl.handle.net/1822/19930
DOI10.1016/j.colsurfb.2011.09.009
ISSN0927-7765
Publisher versionhttp://www.sciencedirect.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
vaz_2012.pdf328,02 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID