Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/1962

TitleProduction of 6-aminopenicillanic acid in aqueous two-phase systems by recombinant Escherichia coli with intracellular penicillin acylase
Author(s)Cao, Xue-jun
Wu, Xing-yan
Fonseca, Luís Pina
Cabral, J. M. S.
Marcos, João Carlos
Keywords6-aminopenicillanic acid production
Aqueous two-phase systems
Bioconversion
Recombinant E. coli A56
Penicillin acylase
Issue date2004
PublisherKluwer
JournalBiotechnology Letters
Citation"Biotechnology letters". 26 (2004) 97-101.
Abstract(s)Bioconversion of Penicillin G in PEG 20000-Dextran T 70 aqueous two-phase systems was achieved using the recombinant Escherichia coli A56 (ppA22) with intracellular penicillin acylase as catalyst. The best conversion conditions were attained for: 7%(w/v) substrate (penicillin G), enzyme activity in bottom phase 52 U/ml, pH 7.8, temperature 37°C, reaction time 40 min. Five repeated batches could be performed in these conditions. Conversions ratios between 0.902-0.985mol of 6-aminopenicillanic acid (6-APA) per mol of penicillin G, were obtained and specific productivity was 3.6-4.6 μmol/min•ml. In addition the product 6-APA could directly be crystallized from the top phase with a purity of 96.2%.
TypeArticle
URIhttp://hdl.handle.net/1822/1962
DOI10.1023/B:BILE.0000012885.62462.f3
ISSN0141-5492
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CDQuim - Artigos (Papers)

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