Please use this identifier to cite or link to this item:

TitleProduction of 6-aminopenicillanic acid in aqueous two-phase systems by recombinant Escherichia coli with intracellular penicillin acylase
Author(s)Cao, Xue-jun
Wu, Xing-yan
Fonseca, Luís Pina
Cabral, J. M. S.
Marcos, João Carlos
Keywords6-aminopenicillanic acid production
Aqueous two-phase systems
Recombinant E. coli A56
Penicillin acylase
Issue date2004
JournalBiotechnology Letters
Citation"Biotechnology letters". 26 (2004) 97-101.
Abstract(s)Bioconversion of Penicillin G in PEG 20000-Dextran T 70 aqueous two-phase systems was achieved using the recombinant Escherichia coli A56 (ppA22) with intracellular penicillin acylase as catalyst. The best conversion conditions were attained for: 7%(w/v) substrate (penicillin G), enzyme activity in bottom phase 52 U/ml, pH 7.8, temperature 37°C, reaction time 40 min. Five repeated batches could be performed in these conditions. Conversions ratios between 0.902-0.985mol of 6-aminopenicillanic acid (6-APA) per mol of penicillin G, were obtained and specific productivity was 3.6-4.6 μmol/min•ml. In addition the product 6-APA could directly be crystallized from the top phase with a purity of 96.2%.
AccessOpen access
Appears in Collections:CDQuim - Artigos (Papers)

Files in This Item:
File Description SizeFormat 
ArtPenProd_RepUM.pdf157,68 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID