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TitleThe bacterial exotoxin AIP56 induces fish macrophage and neutrophil apoptosis using mechanisms of the extrinsic and intrinsic pathways
Author(s)Ramos, Carolina Costa
Vale, Ana do
Ludovico, Paula
Santos, Nuno M. S.
Silva, Manuel T.
KeywordsPhotobacterium damselae ssp. piscicida
Issue dateJan-2011
JournalFish & Shellfish Immunology
Abstract(s)It has been previously shown that the exotoxin of the important fish pathogen Photobacterium damselae ssp. piscicida is a key pathogenicity factor and is responsible for the extensive systemic apoptosis of macrophages and neutrophils seen in acute fish photobacteriosis. The focus of the present study was to further characterize the AIP56-induced apoptosis of sea bass professional phagocytes by assessing the involvement of caspases, mitochondria and oxidative stress. The resulting data indicate that the apoptotic response in peritoneal macrophages and neutrophils treated ex vivo with AIP56 involves activation of caspase-8, -9 and -3, and mitochondria as shown by loss of mitochondrial membrane potential, release of cytochrome c and over-production of ROS. These results together with previous data from this laboratory suggest that both the extrinsic and intrinsic apoptotic athways are involved in the AIP56-induced hagocyte apoptosis.
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Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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